Mid Of Term Two Examinationd Biology Department Senior Five Biology Paper Three Practical

“ The Future Belongs To Those Who Believe In The Beauty Of Their Dreams” Eleaner Roosevelt NAME…………………………………………………………………… ……………………………… …COMB…… ………………. MID OF TERM TWO EXAMINATIONS BIOLOGY DEPARTMENT S.5 BIOLOGY PAPER THREE(03) TOPICS:CELL BIOLOGY AND NUTRITION IN GREEN PLANTS TIME: 11/2 HOURS INSTRUCTIONS • Attempt all items • Analyze the scenario critically before re sponding • Be precise and straight to the point 1. In a laboratory experiment, a group of biology students isolated chloroplasts from spinach leaves and exposed them to light under controlled conditions. The setup included a light source and a suspension medium with ADP and inorganic phosphate (Pi), but no NADP⁺ was added. After some time, ATP production was detected, but there was no evidence of NADPH formation or oxygen release. “ The Future Belongs To Those Who Believe In The Beauty Of Their Dreams” Eleaner Roosevelt Tasks: a) Based on the experimental setup, identify the type of photophosphorylation taking place. Give reasons for your response (05 scores) …………………………………………………………………………………………………………………………………………… …………………………………………………………………………………………………………………………………………… …………………………………………………………………………………………………………………………………………… …………………………………………………………………………………………………………………………………………… …………………………………………………………………………………………………………………… ………………… …… …………………………………………………………………………………………………………………………………………… …………………………………………………………………………………………………………………………………………… …………………………………………………………………………………………………………………………………………… b) Explain why no oxygen or NADPH was produced during the experiment (04 scores) ……………………………………………………………………………………………………………………………………………………… ……………………………………………………………………………………………………………………………………………………… ……………………………………………………………………………………………………………………………………………………… ……………………………………………………………………………………………………………………………………………………… ………………………………………………………………………… …………………………………………………………………………… …………………………………………………………………………………………………………………………………………………….. “ The Future Belongs To Those Who Believe In The Beauty Of Their Dreams” Eleaner Roosevelt c) Outline the pathway of electron flow in this process and name the key photosystem involved. (06 scores) ……………………………………………………………………………………………………………………………………………………… ……………………………………………………………………………………………………………………………………………………… ……………………………………………………………………………………………………………………………………………………… ……………………………………………………………………………………………………………………………………………………… ………………………………………………………………………… …………………………………………………………………………… ……………………………………………………………………………………………………………………………………………………… ………………………………………………………………………………………………………………………………………………… …… ……………………………………………………………………………………………………………………………………………………… …………………………………………………………………………………………………………………………………………………… … ……………………………………………………………………………………………………………………………………………………… ……………………………………………………………………………………………………………………………………………………… ……………………………………………………………………………………………………………………………………………………… d) Why is this process important to the plant under certain conditions? (01 scores) ……………………………………………………………………………………………………………………………………………………… ……………………………………………………………………………………………………………………………………………………… ……………………………………………………………………………………………………………………………………………………… ……………………………………………………………………………………………………………………………………………………… e) If NADP ⁺ was suddenly added to the same experimental setup, how would the type of photophosphorylation change? Explain the expected changes in: “ The Future Belongs To Those Who Believe In The Beauty Of Their Dreams” Eleaner Roosevelt i. …